Review



ubch5a e2  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    R&D Systems ubch5a e2
    Ubch5a E2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ubch5a e2/product/R&D Systems
    Average 95 stars, based on 70 article reviews
    ubch5a e2 - by Bioz Stars, 2026-05
    95/100 stars

    Images



    Similar Products

    ubch5a e2  (R&D Systems)
    95
    R&D Systems ubch5a e2
    Ubch5a E2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ubch5a e2/product/R&D Systems
    Average 95 stars, based on 1 article reviews
    ubch5a e2 - by Bioz Stars, 2026-05
    95/100 stars
    		  Buy from Supplier
    e2 e2 616 r d systems  (R&D Systems)
    95
    R&D Systems e2 e2 616 r d systems
    E2 E2 616 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e2 e2 616 r d systems/product/R&D Systems
    Average 95 stars, based on 1 article reviews
    e2 e2 616 r d systems - by Bioz Stars, 2026-05
    95/100 stars
    		  Buy from Supplier
    ha ubiquitin r d systems cat  (R&D Systems)
    95
    R&D Systems ha ubiquitin r d systems cat
    Ha Ubiquitin R D Systems Cat, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ha ubiquitin r d systems cat/product/R&D Systems
    Average 95 stars, based on 1 article reviews
    ha ubiquitin r d systems cat - by Bioz Stars, 2026-05
    95/100 stars
    		  Buy from Supplier
    e2 ubch5a ube2d1 e2 616 r d system  (R&D Systems)
    95
    R&D Systems e2 ubch5a ube2d1 e2 616 r d system
    E2 Ubch5a Ube2d1 E2 616 R D System, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e2 ubch5a ube2d1 e2 616 r d system/product/R&D Systems
    Average 95 stars, based on 1 article reviews
    e2 ubch5a ube2d1 e2 616 r d system - by Bioz Stars, 2026-05
    95/100 stars
    		  Buy from Supplier
    e2 616 100 recombinant human cul1 rbx1 neddylated complex protei r d system  (R&D Systems)
    95
    R&D Systems e2 616 100 recombinant human cul1 rbx1 neddylated complex protei r d system
    E2 616 100 Recombinant Human Cul1 Rbx1 Neddylated Complex Protei R D System, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e2 616 100 recombinant human cul1 rbx1 neddylated complex protei r d system/product/R&D Systems
    Average 95 stars, based on 1 article reviews
    e2 616 100 recombinant human cul1 rbx1 neddylated complex protei r d system - by Bioz Stars, 2026-05
    95/100 stars
    		  Buy from Supplier
    human ubch5a ube2d1 protein r d system  (R&D Systems)
    95
    R&D Systems human ubch5a ube2d1 protein r d system
    Human Ubch5a Ube2d1 Protein R D System, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human ubch5a ube2d1 protein r d system/product/R&D Systems
    Average 95 stars, based on 1 article reviews
    human ubch5a ube2d1 protein r d system - by Bioz Stars, 2026-05
    95/100 stars
    		  Buy from Supplier
    aavrg hsyn mcherry  (Addgene inc)
    93
    Addgene inc aavrg hsyn mcherry
    Aavrg Hsyn Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aavrg hsyn mcherry/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    aavrg hsyn mcherry - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier
    ubch5a  (Addgene inc)
    93
    Addgene inc ubch5a
    a Schematic illustration of the catalytic mechanisms employed by major E3 ligase families: RING, HECT, and RBR. Acceptor lysine (on E3 or on substrate) is shown in orange. b Schematic showing the experimental workflow of the E2 discharge assay using free amino acids. c E2 discharge assay using UbcH8∼ISG15 in the presence of various amino acids (50 mM) d E2 discharge assay using UbcH8∼ISG15 in the presence of increasing concentrations of L-lys as indicated. e E2 discharge assay using UbcH7∼Ub in the presence of various amino acids (50 mM f E2 discharge assay using <t>UbcH5A∼Ub</t> in the presence of various amino acids (50 mM)
    Ubch5a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ubch5a/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    ubch5a - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier
    joe landry rrid addgene 42630 pproex hta ubch5a aftp0163  (Addgene inc)
    93
    Addgene inc joe landry rrid addgene 42630 pproex hta ubch5a aftp0163
    a Schematic illustration of the catalytic mechanisms employed by major E3 ligase families: RING, HECT, and RBR. Acceptor lysine (on E3 or on substrate) is shown in orange. b Schematic showing the experimental workflow of the E2 discharge assay using free amino acids. c E2 discharge assay using UbcH8∼ISG15 in the presence of various amino acids (50 mM) d E2 discharge assay using UbcH8∼ISG15 in the presence of increasing concentrations of L-lys as indicated. e E2 discharge assay using UbcH7∼Ub in the presence of various amino acids (50 mM f E2 discharge assay using <t>UbcH5A∼Ub</t> in the presence of various amino acids (50 mM)
    Joe Landry Rrid Addgene 42630 Pproex Hta Ubch5a Aftp0163, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/joe landry rrid addgene 42630 pproex hta ubch5a aftp0163/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    joe landry rrid addgene 42630 pproex hta ubch5a aftp0163 - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    a Schematic illustration of the catalytic mechanisms employed by major E3 ligase families: RING, HECT, and RBR. Acceptor lysine (on E3 or on substrate) is shown in orange. b Schematic showing the experimental workflow of the E2 discharge assay using free amino acids. c E2 discharge assay using UbcH8∼ISG15 in the presence of various amino acids (50 mM) d E2 discharge assay using UbcH8∼ISG15 in the presence of increasing concentrations of L-lys as indicated. e E2 discharge assay using UbcH7∼Ub in the presence of various amino acids (50 mM f E2 discharge assay using UbcH5A∼Ub in the presence of various amino acids (50 mM)

    Journal: bioRxiv

    Article Title: ISGylation Mechanism Uncovers Conformational Specificity for HECT-family E3 ligase

    doi: 10.1101/2025.09.26.678755

    Figure Lengend Snippet: a Schematic illustration of the catalytic mechanisms employed by major E3 ligase families: RING, HECT, and RBR. Acceptor lysine (on E3 or on substrate) is shown in orange. b Schematic showing the experimental workflow of the E2 discharge assay using free amino acids. c E2 discharge assay using UbcH8∼ISG15 in the presence of various amino acids (50 mM) d E2 discharge assay using UbcH8∼ISG15 in the presence of increasing concentrations of L-lys as indicated. e E2 discharge assay using UbcH7∼Ub in the presence of various amino acids (50 mM f E2 discharge assay using UbcH5A∼Ub in the presence of various amino acids (50 mM)

    Article Snippet: The following plasmids were obtained from Addgene: UBE1 (Addgene plasmid #34965; gift from Cynthia Wolberger), UbcH5A (Addgene plasmid #15782; gift from Wade Harper), and NEDD4L (Addgene plasmid #165103; gift from Arthur Haas).

    Techniques: